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Recombinant Human KGF/FGF7 HQ

Recombinant Human KGF/FGF7 HQ

Hersteller	Cell Sciences
Kategorie	Oncology Neuroscience Targets for Rodent Neurosciences By Cell Type Carcinoma Hematology Myeloma Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton Intermediate filaments
Typ	Proteins
Specific against	Human
Menge	10 ug
ArtNr	CS-CRK107A
Targets	FGF7
eClass 6.1	34160400
eClass 9.0	42020190
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Biological Activity	Bioactivity is assayed by measuring the dose dependent effect of human KGF on proliferation of mouse BaF3 transfected with FGF3 receptor. The ED50 for this batch of recombinant human KGF is typically below 4 ng/ml.
Description	Keratinocyte Growth Factor (KGF, FGF-7, HBGF-7) is a member of the FGF family of 23 related mitogenic proteins. These proteins play a central role during embryonic development and postnatal growth and tissue repair, by promoting cellular proliferation and differentiation. KGF specifically stimulates epithelial cells and keratinocytes, it is the most potent growth factor identified thus far for skin keratinocytes. KGF takes part in the development of kidney and lung, angiogenesis and has a key role in wound healing following skin injuries. It shows considerable species cross-reactivity (mouse, monkey, pig cells) as the other members of the FGF family.
Endotoxin Level	< 0.005 ng per ug of product (0.05 EU/ug) as measured by turbidimetric kinetic assay.
Formulation	Lyophilized from a 0.2 um sterile filtered solution of PBS, pH 7.2.
Molecular Weight	Recombinant human KGF contains 164 amino acids and a 16 a.a. histidine-based tag for a total length of 180 a.a. and has a predicted MW = 21.2 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent MW = 30 and 38 kDa in SDS-PAGE..
Reconstitution	Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). The optimal concentration should be determined by the user for each specific application.
Storage and Stability	The lyophilized protein, though stable at room temperature for two weeks, is best stored at -20C. Reconstituted protein should be used immediately or stored in working aliquots at -20C. Avoid repeated freeze-thaw cycles.
Specificity	> 2.5 x 10E5 U/mg

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