Recombinant Human KGF/FGF7 HQ

The lyophilized protein, though stable at room temperature for two weeks, is best stored at -20C. Reconstituted protein should be used immediately or stored in working aliquots at -20C. Avoid repeated freeze-thaw cycles.

Keratinocyte Growth Factor (KGF, FGF-7, HBGF-7) is a member of the FGF family of 23 related mitogenic proteins. These proteins play a central role during embryonic development and postnatal growth and tissue repair, by promoting cellular proliferation and differentiation. KGF specifically stimulates epithelial cells and keratinocytes, it is the most potent growth factor identified thus far for skin keratinocytes. KGF takes part in the development of kidney and lung, angiogenesis and has a key role in wound healing following skin injuries. It shows considerable species cross-reactivity (mouse, monkey, pig cells) as the other members of the FGF family.

Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). The optimal concentration should be determined by the user for each specific application.

Bioactivity is assayed by measuring the dose dependent effect of human KGF on proliferation of mouse BaF3 transfected with FGF3 receptor. The ED50 for this batch of recombinant human KGF is typically below 4 ng/ml.