Recombinant Human M-CSF/CSF1 HQ

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 10 ug
ArtNr CS-CRM146A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological ativity on mouse and rat cells and although mouse M-CSF is active in rat cells it is inactive in human cells
Formulation
Lyophilized from PBS, pH 7.2, sterile filtered through a 0.2 um filter.
Reconstitution
Centrifuge vial prior to opening. Reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 ug/ml. The solution can be further diluted into aliquots in aqueous buffers. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). The optimal concentration should be determined by the user for each specific application.
Storage and Stability
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20C. Reconstituted protein should be used immediately or stored in working aliquots at -20C. Avoid repeated freeze-thaw cycles. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed.
GeneID
1435
Source
Recombinant Human M-CSF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than, E. coli, or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize cells in culture.
Molecular Weight
Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175 a.a. and has a predicted MW = 20.7 kDa. The recombinant protein migrates with an apparent MW = 24-26 kDa in SDS-PAGE.
Purity
> 95% by SDS-PAGE gel analysis.
Specific Activity
> 5 x 10E5 U/mg
Endotoxin Level
Endotoxin level is less than 0.005 ng per ug. (0.05 EU/ug) as measured by turbidimetric kinetic assay.
Biological Activity
Bioactivity is assayed by measuring the dose-dependent effect of human M-CSF on proliferation of M-NFS-60 cells. The ED50 value is typically <2 ng/ml.

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Menge: 10 ug
Lieferbar: In stock
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