Manufacturer |
Cell Sciences
|
Category |
|
Type |
Proteins |
Specific against |
Human |
Amount |
10 ug |
Item no. |
CS-CRM146A |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Biological Activity |
Bioactivity is assayed by measuring the dose-dependent effect of human M-CSF on proliferation of M-NFS-60 cells. The ED50 value is typically <2 ng/ml. |
Description |
M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological ativity on mouse and rat cells and although mouse M-CSF is active in rat cells it is inactive in human cells |
Endotoxin Level |
Endotoxin level is less than 0.005 ng per ug. (0.05 EU/ug) as measured by turbidimetric kinetic assay. |
Formulation |
Lyophilized from PBS, pH 7.2, sterile filtered through a 0.2 um filter. |
Molecular Weight |
Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175 a.a. and has a predicted MW = 20.7 kDa. The recombinant protein migrates with an apparent MW = 24-26 kDa in SDS-PAGE. |
Reconstitution |
Centrifuge vial prior to opening. Reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 ug/ml. The solution can be further diluted into aliquots in aqueous buffers. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). The optimal concentration should be determined by the user for each specific application. |
Storage and Stability |
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20C. Reconstituted protein should be used immediately or stored in working aliquots at -20C. Avoid repeated freeze-thaw cycles. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. |
Specificity |
> 5 x 10E5 U/mg |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.