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Recombinant Human PDGF-BB

ArtNr CS-CRP302C
Hersteller Cell Sciences
Menge 1.0 mg
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Host E.coli
Purity >95.0% as determined by reducing and non-reducing SDS-PAGE and UV spectroscopy at 280 nm
Sequence SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIGIVRK KPIFKKATVT LGDHLACKCE TVAAARPVT
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products PDGF, PDGF2, SIS, Platelet-derived growth factor, B chain, FLJ12858, SSV, c-sis, beta polypeptide (oncogene SIS), becaplermin, platelet-derived growth factor 2, platelet-derived growth factor beta, oncogene SIS, platelet-derived growth factor, v-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog)
Lieferbar
Manufacturer - Category
Biomolecules
Storage Conditions
Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
Molecular Weight
ca.24.3 kDa.
Description
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF. Recombinant Human PDGF-BB is a non-glycosylated, disulfide-linked homodimer containing two 109 amino acid chains.
Formulation
Recombinant human PDGF-AA is lyophilized from 10 mM acetic acid (AcOH).
Reconstitution
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Biological Activity
The activity is determined by the dose-dependent proliferation of 3T3 indicator cells and is typically 1.5-2.2 ng/ml.
Endotoxin Level
Measured by kinetic LAL analysis and is typically <=1 EU/ug protein.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1.0 mg
Lieferbar: In stock
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