Recombinant Human TNF-beta

Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week at 2-4C. For long term storage, aliquot and store at -20C to -80C with a carrier protein such as 0.1% HSA or BSA as a stabilizer. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.

Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Recombinant Human TNF-beta is a single, non-glycosylated, polypeptide chain containing 172 amino acids and purified by proprietary techniques.

Centrifuge vial prior to opening. First add sterile water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.

The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is < 0.05 ng/ml.