Recombinant Rat TNF-alpha

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Rat
Menge 1.0 mg
Host E.coli
ArtNr CS-CRT102C
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Tumor necrosis factor-alpha (TNF-alpha), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-alpha occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Two types of receptors for TNF-alpha have been described and virtually all cell types studied show the presence of one or both of these receptor types. Recombinant Rat TNF-alpha is a single non-glycosylated polypeptide chain containing 157 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH7.2, + 150 mM NaCl.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
24835
Source
E. coli
Molecular Weight
Approximately 17.2 kDa
Purity
>98% by SDS-PAGE and HPLC analyses.
Specific Activity
> 1.0 x 10E7 IU/mg in the presence of actinomycin D.
Endotoxin Level
<1 EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cytotoxicity assay using mouse L929 cells is less than 0.1 ng/ml.
AA Sequence
MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL

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Menge: 1.0 mg
Lieferbar: In stock
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