Recombinant Human VEGF 165

Vascular Endothelial Growth Factor is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase (flt-1), KDR gene product (the mouse homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant Human VEGF 165 is a disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

7422

38.2 kDa

1.3 X 10E5 IU/mg

Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) is less than 8 ng/ml.