Recombinant Human Deoxyribonuclease I

Stable for 2 years at 2-4C and for 3 weeks at 15C, pH 6.3.

Recombinant Human Deoxyribonuclease I is an enzyme that selectively cleaves DNA. It is an endonuclease enzyme that splits phosphodiester linkages within polynucleotides, acting on single stranded DNA, double stranded DNA, and chromatin. DNase is activated by bivalent metals such as Mg++ and Ca++. DNase enzymes are common reagents used in biochemical methods requiring digestion of DNA and recovery of RNA, or where DNAneeds to be removed without affecting structural proteins or enzymes. DNase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments. Recombinant Human DNASE1 is a glycosylated polypeptide chain containing 260 amino acids and purified by proprietary chromatographic techniques.

1000 IU/mg. DNase activity is assayed according to the photometric method of Kunitz. One Kunitz unit results in an increase in absorbance at 260 nm of 0.001/minute at 25C when acting upon highly polymerized solution of DNA at pH 5. In addition, 0.005 Kunitz unit digests 1 ug of lambda phage DNA in 10 minutes at 37C in 50 mM Tris + 1 mM Mg++, pH 7.8, in a 50 ul reaction.