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Recombinant Human UBE2B

ArtNr CS-CSI10743
Hersteller Cell Sciences
Menge 10 ug
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Host E.coli
Purity Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Sequence MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products RAD6B, UBC2, E2-17kDa, HHR6B, HR6B, E2 protein, ubiquitin carrier protein B, ubiquitin-conjugating enzyme E2B, ubiquitin-protein ligase B
Lieferbar
Manufacturer - Category
Biomolecules
Storage Conditions
Lyophilized UBE2B although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution UBE2B should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Description
This E2 enzyme encodes for the human homolog of the yeast DNA repair gene RAD6, which is induced by DNA damaging agents. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner, in vitro , and is essential for the multi-ubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia. Ubiquitin Conjugating Enzyme E2B Human Recombinant produced in, E. coli, is a 19 kDa protein containing 166 amino acids. The UE2B protein contains 6xHis tag and is purified by proprietary chromatographic techniques.
Formulation
Lyophilized from a 0.2 um filtered concentrated (1 mg/ml) solution in 1 X PBS and 1 mM DTT, pH 7.5.
Reconstitution
It is recommended to reconstitute the lyophilized UBE2B in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: In stock
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