Vergleich

Recombinant Rat TNF-alpha/TNFSF1A

ArtNr CS-CRT102B
Hersteller Cell Sciences
Menge 20 ug
Kategorie
Typ Proteins
Specific against Rat (Rattus norvegicus)
Host E.coli
Purity >98% by SDS-PAGE and HPLC analyses.
Sequence MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products TNF-alpha, TNF, TNF-a, TNFA, TNFSF2, member 2, tumor necrosis factor, TNF superfamily, DADB-70P7.1, DIF, APC1 protein, macrophage-derived, monocyte-derived, cachectin, tumor necrosis factor alpha, tumor necrosis factor ligand superfamily member 2
Lieferbar
Manufacturer - Category
Biomolecules
Storage Conditions
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
Molecular Weight
Approximately 17.2 kDa
Description
Tumor necrosis factor-alpha (TNF-alpha), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-alpha occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Two types of receptors for TNF-alpha have been described and virtually all cell types studied show the presence of one or both of these receptor types. Recombinant Rat TNF-alpha is a single non-glycosylated polypeptide chain containing 157 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH7.2, + 150 mM NaCl.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Specificity
> 1.0 x 10E7 IU/mg in the presence of actinomycin D.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cytotoxicity assay using mouse L929 cells is less than 0.1 ng/ml.
Endotoxin Level
<1 EU/ug as determined by LAL method.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 20 ug
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen