Recombinant Rat TNF-alpha/TNFSF1A

Tumor necrosis factor-alpha (TNF-alpha), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-alpha occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Two types of receptors for TNF-alpha have been described and virtually all cell types studied show the presence of one or both of these receptor types. Recombinant Rat TNF-alpha is a single non-glycosylated polypeptide chain containing 157 amino acids.

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH7.2, + 150 mM NaCl.

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

> 1.0 x 10E7 IU/mg in the presence of actinomycin D.