|
Hersteller |
Cell Sciences
|
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Rat |
|
Menge |
20 ug |
|
Host |
E.coli |
|
ArtNr |
CS-CRT102B |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Description |
Tumor necrosis factor-alpha (TNF-alpha), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-alpha occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Two types of receptors for TNF-alpha have been described and virtually all cell types studied show the presence of one or both of these receptor types. Recombinant Rat TNF-alpha is a single non-glycosylated polypeptide chain containing 157 amino acids. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH7.2, + 150 mM NaCl. |
| Reconstitution |
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions. |
| Storage and Stability |
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles. |
| GeneID |
24835 |
| Source |
E. coli |
| Molecular Weight |
Approximately 17.2 kDa |
| Purity |
>98% by SDS-PAGE and HPLC analyses. |
| Specific Activity |
> 1.0 x 10E7 IU/mg in the presence of actinomycin D. |
| Endotoxin Level |
<1 EU/ug as determined by LAL method. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 determined by a cytotoxicity assay using mouse L929 cells is less than 0.1 ng/ml. |
| AA Sequence |
MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL |
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