Item no. |
CS-CRT102B |
Manufacturer |
Cell Sciences
|
Amount |
20 ug |
Category |
|
Type |
Proteins |
Specific against |
Rat (Rattus norvegicus) |
Host |
E.coli |
Purity |
>98% by SDS-PAGE and HPLC analyses. |
Sequence |
MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Similar products |
TNF-alpha, TNF, TNF-a, TNFA, TNFSF2, member 2, tumor necrosis factor, TNF superfamily, DADB-70P7.1, DIF, APC1 protein, macrophage-derived, monocyte-derived, cachectin, tumor necrosis factor alpha, tumor necrosis factor ligand superfamily member 2 |
Available |
|
Manufacturer - Category |
Biomolecules |
Storage Conditions |
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles. |
Molecular Weight |
Approximately 17.2 kDa |
Description |
Tumor necrosis factor-alpha (TNF-alpha), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-alpha occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Two types of receptors for TNF-alpha have been described and virtually all cell types studied show the presence of one or both of these receptor types. Recombinant Rat TNF-alpha is a single non-glycosylated polypeptide chain containing 157 amino acids. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH7.2, + 150 mM NaCl. |
Reconstitution |
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions. |
Specificity |
> 1.0 x 10E7 IU/mg in the presence of actinomycin D. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 determined by a cytotoxicity assay using mouse L929 cells is less than 0.1 ng/ml. |
Endotoxin Level |
<1 EU/ug as determined by LAL method. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.