FAMPAC Cells

The Fampac cell line was established from the primary pancreatic adenocarcinoma of an adult female who had a familial predisposition to pancreatic cancer. These cells are epithelial in nature and have been utilized extensively in research focused on the biological behavior of pancreatic cancer, including studies on tumor progression, metastasis, and therapeutic response. The Fampac cell line is known for its aggressive tumor-forming capability in xenograft models, which makes it valuable for in vivo studies related to drug efficacy and cancer cell biology.
In vitro, Fampac cells exhibit characteristics typical of pancreatic adenocarcinoma, including resistance to apoptosis and the ability to proliferate under chemically defined conditions. This resistance to programmed cell death is a critical feature for studies looking to explore new chemotherapeutic agents and their potential to induce cancer cell death. Additionally, Fampac cells have been used to study the molecular mechanisms of pancreatic cancer pathogenesis, offering insights into genetic mutations, signaling pathways involved in cancer proliferation, and interactions with the tumor microenvironment.

Adherent

43 years

Caucasian

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes, in nude mice, adenocarcinoma

1 x 10^4 cells/cm^2 will yield in a confluent layer in about 2to3 days

FAMPAC cells carry a homozygous Kras mutation in codon12: GGT(Gly) |BiggerAs|GTT(Val)

45-48, X, +3, -5, +der(5), +der(5), +der(5)add(p14), -7, +10, +2der(10)add(p15)add(q26), der(12)add(p13), der(12)add(p11), -13, -13, +der(13)add(p11), -14, der?(14), -15, i(15q), der(16)(q+), -19, -20, -21, -22, +3-5mar