SK-MES-1 Cells

ArtNr	CLS-300339
Hersteller	CLS Cell Lines Service
Menge	1 cryovial
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Zertifikat	The certificate of analysis can be requested on the website or via email at info@cytion.com. Please indicate the lot number of your product in the email.
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	SK MES 1, SKMES-1, SK-Mes-1, SK-MES1, SKMES1, SK-MES, SKMES
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Manufacturer - Category	Lung cancer cell lines
Description	SK-MES-1 is a human lung squamous cell carcinoma (LSQCC) cell line extensively used in lung cancer research, particularly in studies focusing on the second most common subtype of non-small cell lung cancer (NSCLC). SK-MES-1 cells are characterized by a high mutation rate in the tumor suppressor gene p53, which is implicated in their resistance to apoptosis and various chemotherapies. This cell line serves as an important model for evaluating novel therapeutic strategies against lung squamous cell carcinoma, particularly for drugs that target the cell cycle and apoptotic pathways. Studies involving SK-MES-1 have shown that the cell line is responsive to platinum-based chemotherapy agents, such as lobaplatin, which induce apoptosis via both intrinsic and extrinsic pathways. Lobaplatin, a third-generation platinum compound, has been shown to inhibit SK-MES-1 proliferation by inducing S-phase cell cycle arrest and promoting apoptosis through upregulation of pro-apoptotic proteins like Bax and downregulation of anti-apoptotic proteins such as Bcl-2. Additionally, SK-MES-1 cells treated with lobaplatin exhibited an increase in caspase-3, -8, and -9 activation, further supporting the involvement of mitochondrial-mediated apoptosis. SK-MES-1 has also been used to study the effects of other compounds, such as costunolide, a phytochemical that induces G1/S phase cell cycle arrest and apoptosis via a mitochondria-dependent pathway. Costunolide treatment increases the expression of p53 and Bax, while reducing Bcl-2 levels and disrupting mitochondrial membrane potential, further confirming the utility of SK-MES-1 in studying apoptosis-related pathways in lung squamous carcinoma.
Tissue	Lung
Growth properties	Adherent
Disease	Squamous cell carcinoma
Age	65 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin	X, Y
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Metastatic Site	Pleural effusion
Protein Expression	p53 negative
Karyotype	The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining.
Isoenzymes	Me-2, 1-2, PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B, Phenotype Frequency Product: 0.0132
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 1 cryovial

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