T406 Cells

The T406 cell line is derived from a human glioblastoma multiforme (GBM), a highly aggressive brain tumor classified as WHO Grade IV. This cell line has been extensively studied for its genetic characteristics, particularly the overexpression of the erbB oncogene. Cytogenetic analysis of T406 revealed polysomy of chromosome 7, a common feature in high-grade gliomas, with up to six copies of chromosome 7 present per cell. This polysomy correlates with increased expression of the erbB oncogene, which plays a role in tumor proliferation and survival. The T406 cell line has been used to study the molecular mechanisms of glioblastoma progression and the role of growth factor receptors in tumorigenesis.
T406 has also been included in studies evaluating the heterogeneity of tumor responses to chemoradiotherapy. Research has demonstrated that T406, along with other GBM cell lines, shows variability in the expression of heparanase (HPSE) and heparan sulfate (HS), which are involved in the tumor microenvironment's remodeling. This heterogeneity in expression may contribute to treatment resistance and tumor relapse, making T406 an important model for understanding the effects of therapy on tumor biology. Furthermore, T406 has been used as part of larger panels of glioblastoma models to explore tumor growth and resistance pathways, serving as a critical tool in preclinical cancer research.

Adherent

53 years

Caucasian

1

Supplement the medium with 10% FBS

2 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

A ratio of 1:4 is recommended