|
Hersteller |
GenScript
|
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
other |
|
Menge |
50ug |
|
ArtNr |
Z02963-50 |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Product Line |
Others |
| Description |
Batroxobin is a serine protease derived from the venom of Bothrops atrox. This thrombin-like proteolytic enzyme splits the 16 Arg-17 Gly bond in the A (alpha)-chain of fibrinogen, releasing fibrinopeptide A and leading to the clot formation through aggregation of formed of fibrin I monomer or Des-AA-monomer. In contrast to other anti-coagulants, it does not affect the functions of platelets. Hence, Batroxobin is used to determine fibrinogen in plasma, to measure a 'batroxobin clotting time' as a heparin-insensitive parallel to the thrombin time, to investigate dysfibrinogenemia, and to test the contractile system of platelets. |
| M.W. |
A single, glycosylated, polypeptide chain containing 231 amino acids, and having a molecular mass of approximately 28, 000ca.33, 000. |
| Purity |
>97% by SDS-PAGE and HPLC analyses. |
| Endotoxin Level |
Less than 0.5EU/mg of rRpBX/SVTLE as determined by LAL method. |
| Specific Activity |
Fully biologically active when compared to standard. The biologically activity of rRpBX is no less than 500KU/mg (Klobusitzky Unit). One KU is defined as the amount of enzyme which coagulates standard human plasma incubated at 37 C in vitro within 60+/-20 seconds. |
| Storage |
This lyophilized preparation is stable at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -70C. Avoid repeated freeze/thaw cycles. |
| Formulation |
Lyophilized from a 0.2um filtered concentrated sodium acetate buffer solution. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <=-20C. Further dilutions should be made in appropriate buffered solutions. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Sequence |
VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIHLGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSEHIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVC REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCA EPRKPAFYTK VFDYLPWIQS IIAGNKTATC P |
