KG-1 Cells

KG-1 is a human acute myelogenous leukemia (AML) cell line derived from the bone marrow of an adult patient with erythroleukemia. This cell line is a valuable model for studying hematopoietic differentiation and leukemia, particularly due to its unique characteristics, including the expression of several hematopoietic markers. KG-1 cells are classified as immature myeloid cells that resemble early progenitor cells, which makes them a useful tool for investigating the early stages of myeloid lineage commitment and the molecular mechanisms driving leukemogenesis.

KG-1 cells exhibit a high degree of plasticity, which allows them to differentiate into various hematopoietic lineages under the right experimental conditions. This characteristic is particularly important for research in understanding the regulation of hematopoiesis and the development of therapeutic strategies aimed at targeting leukemic stem cells. Additionally, KG-1 cells are known to express markers such as CD34, HLA-DR, and CD13, which are critical in both normal and malignant hematopoiesis, making them an excellent model for flow cytometry and other immunophenotyping studies.

KG-1 has also been employed in drug discovery and toxicity testing, where its responsiveness to differentiation agents and chemotherapeutic drugs can be evaluated. As with all in vitro models, it is important to recognize that KG-1 cells are for research use only and not suitable for therapeutic or in vivo applications.

Suspension

Acute myelogenous leukemia

Male

1

IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a)

Transfer the cell suspension into sterile centrifuge tubes. Collect the cells by spinning down at 300xg for 3 minutes. Discard the supernatant and resuspend the pelleted cells in fresh cell culture medium. Adjust to an optimal cell density between 1 - 3 x 10^5 cells/ml. Split the cells when a maximum cell density of 1 - 2 x 10^6 cells/ml is reached.

Allow the cells to recover from the freezing process for at least 24 hours.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

A ratio of 1:2 is recommended

HLA A30, A31, B35, Cw4

G6PD, B, PGM1, 1-2, PGM3, 0, ES-D, 1, Me-2, 1, AK-1, 0, GLO-1, 2