Human Mesenchymal Stem Cells - Chorion Villi

Drug testing, regenerative medicine, disease research

Human Mesenchymal Stem Cells (MSCs) derived from chorion villi represent a highly versatile population of multipotent stromal cells capable of differentiating into multiple lineages, including adipocytes, osteoblasts, and chondrocytes. These cells are isolated from the chorionic villi, a part of the placenta that plays a critical role in maternal-fetal exchange. Chorion villi are unique in that they are composed of both fetal and maternal tissues, providing a distinct microenvironment that contributes to the robust self-renewing and differentiation capabilities of the MSCs derived from this source. The MSCs from chorion villi exhibit a more primitive phenotype compared to MSCs derived from adult tissues, often displaying a higher proliferation rate and broader differentiation potential. These characteristics make them particularly valuable for research in regenerative medicine, tissue engineering, and disease modeling.

These MSCs have been rigorously demonstrated in vitro to differentiate into adipocytes, osteoblasts, and chondrocytes when cultured in lineage-specific differentiation media, underscoring their potential for applications in tissue regeneration and disease modeling. The unique origin of these cells from the chorionic villi imparts them with specific immunomodulatory properties, which may differ from MSCs derived from other sources such as bone marrow or adipose tissue. This distinction is crucial for studies focusing on immune-related conditions or developing allogeneic cell therapies.

MSCs are cryopreserved at early passages in a specialized cryomedium, ensuring their viability and functionality post-thaw. Each cryovial contains a minimum of 1 x 106 cells with a viability rate ranging between 92% and 95%, as determined by the Trypan Blue dye exclusion test. These cells are sourced from healthy donors who have provided informed consent, ensuring ethical collection practices. Each batch undergoes stringent quality control assessments, including thorough testing for cell identification, purity, potency, and viability. These measures guarantee that the cultured MSCs are of high quality and appropriate for research applications, excluding therapeutic or in vivo use.

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Caucasian

1

Alpha MEM, w: 2.0 mM stable Glutamine, w/o: Ribonucleosides, w/o: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

1 to 3 x 10^3 cells/cm^2

Trypsin-EDTA