Hersteller |
CLS Cell Lines Service
|
Kategorie |
|
Typ |
Cell line |
Specific against |
other |
Menge |
1 cell culture flask |
ArtNr |
CLS-330144 |
eClass 6.1 |
32160890 |
eClass 9.0 |
32160890 |
Lieferbar |
|
age |
56 years |
gender |
Male |
ethnicity |
Caucasian |
morphology |
Polygonal |
growthProperties |
Adherent; colonies |
citation |
Capan-2 (CLS catalog number 300144) |
biosafetyLevel |
1 |
proteinExpression |
p53 negative |
antigenExpression |
Blood Type B; Rh+ |
isoenzymes |
Me-2, 2; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 2; Phenotype Frequency Product: 0.0004 |
tumorigenic |
Yes, in nude mice; forms well differentiated adenocarcinoma consistent with pancreatic carcinoma |
products |
mucin (apomucin, MUC-1, MUC-2) |
ploidyStatus |
Aneuploid |
mutationalProfile |
Capan-2 cells carry a heterozygous Kras mutation in codon12: GGT> GTT |
cultureMedium |
McCoys 5A |
mediumSupplements |
10% FBS, 2 mM L-glutamine |
doublingTime |
45-60 hours |
subculturing |
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
splitRatio |
A ratio of 1:3 to 1:6 is recommended |
seedingDensity |
1 x 10^4 cells/cm^2 will result in a confluent monolayer within 7 days. |
fluidRenewal |
2 - 3 times per week |
freezingRecovery |
After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
freezeMedium |
CM-ACF (CLS catalog number 800650) |
amelogenin |
X, X |
CSF1PO |
11, 12 |
D13S317 |
11, 12 |
D16S539 |
9, 13 |
D5S818 |
11, 12 |
D7S820 |
9, 11 |
TH01 |
44629 |
TPOX |
8 |
vWA |
17 |
D3S1358 |
17, 18 |
D21S11 |
31 |
D18S51 |
13 |
pentaE |
11 |
pentaD |
13, 15 |
D8S1179 |
12, 13 |
FGA |
21, 24 |
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