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Capan-2 growing culture Europäischer Partner

ArtNr CLS-330144
Hersteller CLS Cell Lines Service
Menge 1 cell culture flask
Kategorie
Typ Cell line
Specific against other
Dry ice Yes
ECLASS 10.1 42040401
ECLASS 11.0 42040401
UNSPSC 41106509
Alias CaPan-2, CAPAN-2, Capan 2, CAPAN 2, Capan2, CAPAN2
Lieferbar
Growth properties
Adherent; colonies
Age
56 years
Gender
Male
Ethnicity
Caucasian
Morphology
Polygonal
Biosafety Level
1
Culture Medium
McCoys 5A
Medium Supplements
10% FBS, 2 mM L-glutamine
Subculturing
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal
2 - 3 times per week
Freezing Recovery
After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Freeze Medium
CM-ACF (CLS catalog number 800650)
Amelogenin
X, X
Tumorigenic
Yes, in nude mice; forms well differentiated adenocarcinoma consistent with pancreatic carcinoma
Split Ratio
A ratio of 1:3 to 1:6 is recommended
Seeding Density
1 x 10^4 cells/cm^2 will result in a confluent monolayer within 7 days.
Doubling Time
45-60 hours
Antigen Expression
Blood Type B; Rh+
Ploidy Status
Aneuploid
Mutational Profile
Capan-2 cells carry a heterozygous Kras mutation in codon12: GGT> GTT
Products
mucin (apomucin, MUC-1, MUC-2)
Protein Expression
p53 negative
Isoenzymes
Me-2, 2; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 2; Phenotype Frequency Product: 0.0004
Manufacturer - Citation
Capan-2 (CLS catalog number 300144)
CSF1PO
11, 12
D13S317
11, 12
D16S539
9, 13
D5S818
11, 12
D7S820
9, 11
TH01
44629
TPOX
8
vWA
17
D3S1358
17, 18
D21S11
31
D18S51
13
pentaE
11
pentaD
13, 15
D8S1179
12, 13
FGA
21, 24

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 cell culture flask
Lieferbar: In stock
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