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Capan-2 growing culture

Capan-2 growing culture

Item no.	CLS-330144
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CaPan-2, CAPAN-2, Capan 2, CAPAN 2, Capan2, CAPAN2
Available
Growth properties	Adherent; colonies
Age	56 years
Gender	Male
Ethnicity	Caucasian
Morphology	Polygonal
Biosafety Level	1
Culture Medium	McCoys 5A
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Freeze Medium	CM-ACF (CLS catalog number 800650)
Amelogenin	X, X
Tumorigenic	Yes, in nude mice; forms well differentiated adenocarcinoma consistent with pancreatic carcinoma
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2 will result in a confluent monolayer within 7 days.
Doubling Time	45-60 hours
Antigen Expression	Blood Type B; Rh+
Ploidy Status	Aneuploid
Mutational Profile	Capan-2 cells carry a heterozygous Kras mutation in codon12: GGT> GTT
Products	mucin (apomucin, MUC-1, MUC-2)
Protein Expression	p53 negative
Isoenzymes	Me-2, 2; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 2; Phenotype Frequency Product: 0.0004
Manufacturer - Citation	Capan-2 (CLS catalog number 300144)
CSF1PO	11, 12
D13S317	11, 12
D16S539	9, 13
D5S818	11, 12
D7S820	9, 11
TH01	44629
TPOX	8
vWA	17
D3S1358	17, 18
D21S11	31
D18S51	13
pentaE	11
pentaD	13, 15
D8S1179	12, 13
FGA	21, 24

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 5/29/2025

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