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Capan-2 growing culture

Capan-2 growing culture

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Amount	1 cell culture flask
Item no.	CLS-330144
eClass 6.1	32160890
eClass 9.0	32160890
Available
age	56 years
gender	Male
ethnicity	Caucasian
morphology	Polygonal
growthProperties	Adherent; colonies
citation	Capan-2 (CLS catalog number 300144)
biosafetyLevel	1
proteinExpression	p53 negative
antigenExpression	Blood Type B; Rh+
isoenzymes	Me-2, 2; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 2; Phenotype Frequency Product: 0.0004
tumorigenic	Yes, in nude mice; forms well differentiated adenocarcinoma consistent with pancreatic carcinoma
products	mucin (apomucin, MUC-1, MUC-2)
ploidyStatus	Aneuploid
mutationalProfile	Capan-2 cells carry a heterozygous Kras mutation in codon12: GGT> GTT
cultureMedium	McCoys 5A
mediumSupplements	10% FBS, 2 mM L-glutamine
doublingTime	45-60 hours
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:3 to 1:6 is recommended
seedingDensity	1 x 10^4 cells/cm^2 will result in a confluent monolayer within 7 days.
fluidRenewal	2 - 3 times per week
freezingRecovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
freezeMedium	CM-ACF (CLS catalog number 800650)
amelogenin	X, X
CSF1PO	11, 12
D13S317	11, 12
D16S539	9, 13
D5S818	11, 12
D7S820	9, 11
TH01	44629
TPOX	8
vWA	17
D3S1358	17, 18
D21S11	31
D18S51	13
pentaE	11
pentaD	13, 15
D8S1179	12, 13
FGA	21, 24

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 5/16/2024