ArtNr |
CLS-330213 |
Hersteller |
CLS Cell Lines Service
|
Menge |
1 cell culture flask |
Kategorie |
|
Typ |
Cell line |
Specific against |
other |
Dry ice |
Yes
|
ECLASS 10.1 |
42040401 |
ECLASS 11.0 |
42040401 |
UNSPSC |
41106509 |
Alias |
HS 683, Hs 683, Hs-683, Hs683, HS683, Hs 683.T, HS 683T, Hs683T |
Lieferbar |
|
Growth properties |
Adherent |
Age |
76 years |
Gender |
Male |
Ethnicity |
Caucasian |
Morphology |
Fibroblast-like |
Biosafety Level |
1 |
Culture Medium |
DMEM |
Medium Supplements |
10% FBS, 2 mM L-glutamine, 4.5 g/L glucose |
Subculturing |
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature (about 20-26°C) for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Fluid Renewal |
Every 3 days |
Freezing Recovery |
After thawing, plate the cells at 4 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze Medium |
CM-1 (CLS catalog number 800150) |
Amelogenin |
X, Y |
Tumorigenic |
No |
Split Ratio |
A ratio of 1:4 is recommended |
Seeding Density |
When seeded at 1 x 10^4 cells/cm^2, the cells will reach 80% confluence within 3-4 d. |
Doubling Time |
45-50 hours |
Ploidy Status |
Aneuploid |
Karyotype |
(P15) hypotetraploid with mode = 88; range = 44 to 97; Y chromosomes present |
Msi Status |
Stable (MSS) |
Isoenzymes |
G6PD, B; PGM1, 1; PGM3, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.0029 |
Manufacturer - Citation |
HS-683 (CLS catalog number 300213) |
CSF1PO |
9, 13 |
D13S317 |
8, 12 |
D16S539 |
9, 1 |
D5S818 |
11, 12 |
D7S820 |
11 |
TH01 |
6, 8 |
TPOX |
8, 11 |
vWA |
18, 2 |
D3S1358 |
14, 16 |
D21S11 |
27, 33.2 |
D18S51 |
12, 14 |
pentaE |
13, 15 |
pentaD |
13, 14 |
D8S1179 |
12, 13 |
FGA |
21.2, 22 |
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