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HS-683 growing culture

HS-683 growing culture

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Amount	1 cell culture flask
Item no.	CLS-330213
eClass 6.1	32160890
eClass 9.0	32160890
Available
age	76 years
gender	Male
ethnicity	Caucasian
morphology	Fibroblast-like
growthProperties	Adherent
citation	HS-683 (CLS catalog number 300213)
biosafetyLevel	1
isoenzymes	G6PD, B; PGM1, 1; PGM3, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.0029
tumorigenic	No
ploidyStatus	Aneuploid
msiStatus	Stable (MSS)
karyotype	(P15) hypotetraploid with mode = 88; range = 44 to 97; Y chromosomes present
cultureMedium	DMEM
mediumSupplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
doublingTime	45-50 hours
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature (about 20-26Â°C) for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:4 is recommended
seedingDensity	When seeded at 1 x 10^4 cells/cm^2, the cells will reach 80% confluence within 3-4 d.
fluidRenewal	Every 3 days
freezingRecovery	After thawing, plate the cells at 4 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
freezeMedium	CM-1 (CLS catalog number 800150)
amelogenin	X, Y
CSF1PO	9, 13
D13S317	8, 12
D16S539	9, 1
D5S818	11, 12
D7S820	11
TH01	6, 8
TPOX	8, 11
vWA	18, 2
D3S1358	14, 16
D21S11	27, 33.2
D18S51	12, 14
pentaE	13, 15
pentaD	13, 14
D8S1179	12, 13
FGA	21.2, 22

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

available

Delivery expected until 5/16/2024