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HS-683 growing culture

HS-683 growing culture

Item no.	CLS-330213
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	HS 683, Hs 683, Hs-683, Hs683, HS683, Hs 683.T, HS 683T, Hs683T
Available
Growth properties	Adherent
Age	76 years
Gender	Male
Ethnicity	Caucasian
Morphology	Fibroblast-like
Biosafety Level	1
Culture Medium	DMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature (about 20-26Â°C) for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	Every 3 days
Freezing Recovery	After thawing, plate the cells at 4 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (CLS catalog number 800150)
Amelogenin	X, Y
Tumorigenic	No
Split Ratio	A ratio of 1:4 is recommended
Seeding Density	When seeded at 1 x 10^4 cells/cm^2, the cells will reach 80% confluence within 3-4 d.
Doubling Time	45-50 hours
Ploidy Status	Aneuploid
Karyotype	(P15) hypotetraploid with mode = 88; range = 44 to 97; Y chromosomes present
Msi Status	Stable (MSS)
Isoenzymes	G6PD, B; PGM1, 1; PGM3, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.0029
Manufacturer - Citation	HS-683 (CLS catalog number 300213)
CSF1PO	9, 13
D13S317	8, 12
D16S539	9, 1
D5S818	11, 12
D7S820	11
TH01	6, 8
TPOX	8, 11
vWA	18, 2
D3S1358	14, 16
D21S11	27, 33.2
D18S51	12, 14
pentaE	13, 15
pentaD	13, 14
D8S1179	12, 13
FGA	21.2, 22

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 5/29/2025

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