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U-2 OS growing culture

U-2 OS growing culture

Item no.	CLS-330364
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	U2OS, U-2OS, U-2-OS, U2-OS, U20-S, U20S, 2T
Available
Growth properties	Monolayer, adherent
Age	15 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	DMEM:Ham' s F12
Medium Supplements	5% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freeze Medium	CM-ACF (CLS catalog number 800650)
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Antigen Expression	Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
Products	Osteosarcoma derived growth factor (ODGF)
Karyotype	(P11-46) hypodiploid to near tetraploid; (P111-118) modal numbers 34 to 37 and 64 to 67 with abnormalities including dicentrics, breaks, rings, and pulverizations plus acrocentric subtelocentric and minute markers
Receptors Expressed	Insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF-I); Osteosarcoma derived growth factor (ODGF)
Isoenzymes	PGM3, 1; PGM1, 2; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0082
Manufacturer - Citation	U-2 OS (CLS catalog number 300364)
CSF1PO	12, 13
D13S317	13
D16S539	11, 12
D5S818	8, 11
D7S820	11, 12
TH01	6, 9.3
TPOX	11, 12
vWA	14, 18
D3S1358	16
D21S11	31
D18S51	12, 14
D8S1179	12, 14
FGA	20
D2S1338	20, 24
D19S433	15

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 5/29/2025

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