ArtNr |
CLS-440104 |
Hersteller |
CLS Cell Lines Service
|
Menge |
1 cell culture flask |
Kategorie |
|
Typ |
Cell line |
Specific against |
other |
Dry ice |
Yes
|
ECLASS 10.1 |
42040401 |
ECLASS 11.0 |
42040401 |
UNSPSC |
41106509 |
Alias |
3T6 Swiss Albino, Swiss 3T6, NIH 3T6, 3T6, GM05862 |
Lieferbar |
|
Growth properties |
Adherent |
Cell type |
Fibroblast |
Age |
Embryo |
Morphology |
Fibroblast-like |
Biosafety Level |
1 |
Viruses |
Negative for ectromelia virus (mousepox). |
Culture Medium |
Ham' s F12 |
Medium Supplements |
10% FBS, 2 mM L-glutamine |
Subculturing |
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Fluid Renewal |
Every 3 to 4 days |
Freezing Recovery |
After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze Medium |
CM-ACF (CLS catalog number 800650) |
Split Ratio |
A ratio of 1:2 to 1:10 is recommended |
Seeding Density |
1 x 10^4 cells/cm^2 will result in a confluent monolayer within 5 days. |
Virus Susceptibility |
Herpes simplex, Vaccinia, Pseudorabies, Vesicular Stomatitis (Indiana) |
Reverse Transcriptase |
Negative |
Products |
Collagen; hyaluronic acid |
Manufacturer - Citation |
3T6-Swiss albino (CLS catalog number 400104) |
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