« Back
Home /
3T6-Swiss albino growing culture

3T6-Swiss albino growing culture

Item no.	CLS-440104
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	3T6 Swiss Albino, Swiss 3T6, NIH 3T6, 3T6, GM05862
Available
Growth properties	Adherent
Cell type	Fibroblast
Age	Embryo
Morphology	Fibroblast-like
Biosafety Level	1
Viruses	Negative for ectromelia virus (mousepox).
Culture Medium	Ham' s F12
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	Every 3 to 4 days
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Freeze Medium	CM-ACF (CLS catalog number 800650)
Split Ratio	A ratio of 1:2 to 1:10 is recommended
Seeding Density	1 x 10^4 cells/cm^2 will result in a confluent monolayer within 5 days.
Virus Susceptibility	Herpes simplex, Vaccinia, Pseudorabies, Vesicular Stomatitis (Indiana)
Reverse Transcriptase	Negative
Products	Collagen; hyaluronic acid
Manufacturer - Citation	3T6-Swiss albino (CLS catalog number 400104)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 1 cell culture flask

available

Delivery expected until 5/29/2025

Express Delivery: Save 3 days

Compare

Add to wishlist

Recommend by mail