Anti Pertussis Toxin

Application: Neutralization, IP, DB, ELISA, WB 
Clonality: Polyclonal 
Host: Rabbit 
Purification: Serum 
Reactivity: Bordetella pertussis 
Perrtussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterra pertussis. PT catalyzes the

ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and

prevents intracellular signal transduction involving the G proteins. PT consists of one moplecule of each S1 (26

kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecule of S4 (12 kDa). This product was highly purified

(> 90% pure) from Bordetellapertussis strain Tohama by the method of Skelton & Wong1). Cytotoxicity of the PT was

confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/ml of PT (see the Figure below). Application: 1. Western blotting (1/2, 000~1/10, 000 dilution)

2. ELISA (1/10, 000~1/20, 000 dilution)



3. Dot blotting (1/2, 000~1/10, 000 dilution)

4. Immunoprecipitation (1/200~1/500 dilution)

5. Neutralising (Assay dependent)

Other applications have not been tested.

Immunogen: Immunization was Initiated with toxoid and boosted with native toxin (BioAcademia 01-503)