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Anti Collagen 4 (Alpha 5) mAb (Clone H52)

Anti Collagen 4 (Alpha 5) mAb (Clone H52)

ArtNr	SGE-C452
Hersteller	Cosmobio
Menge	500 ul
Kategorie	Antibodies Cell Biology Cell Movement Cell Adhesion Western Blot Cell Stainings ECM Primary Antibodies
Typ	Antibody Monoclonal
Applikationen	WB, IF
Clon	H52
Specific against	Human (Homo sapiens), Rat (Rattus norvegicus), Rabbit (Oryctolagus cuniculus), Cattle (Bovine)
Host	Rat
Isotype	IgG2b Kappa
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Lieferbar
Specificity	Domesticated Animal, Rabbit, Bovine, Rat, Human
Manufacturer - Category	Antibodies/Primary Antibodies/Cell Biology/Cytoskeleton; Antibodies/Primary Antibodies/Cell Biology/Extracellular Matrix; Research Area/Cell Biology/Cytoskeleton/Primary Antibodies; Research Area/Cell Biology/Extracellular Matrix/Primary Antibodies; Research Area/Disease/Alport Syndrome/Primary Antibodies; Research Area/Disease/Goodpastures Syndrome/Primary Antibodies; Antibodies/Primary Antibodies/Disease/Alport Syndrome; Antibodies/Primary Antibodies/Disease/Goodpastures Syndrome; Research Area/Organs & Organ Systems/Kidney/Primary Antibodies; Antibodies/Primary Antibodies/Organs & Organ Systems/Kidney
Maker	SGE
Antigen/Source	Collagen 4 (alpha5(4)Chain)
Description	Check out our Human Collagen IVα Rat Monoclonal Antibodies Dashboard for more information about this product and additional epitope-defined monoclonal antibodies useful for research on hereditary diseases related to collagen IV, including our popular Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25) (SGE-CFT45325). Alport syndrome is an inherited disease characterized by the pathological absence or reduction of the collagen α 5(IV) chain in glomerular basement membrane (GBM), tubular basement membrane (TBM) and Bowman's capsular basement membrane. Anti Collagen 4 (Alpha 5) mAb (Clone H52) (cat no. SGE-C452) is an unlabeled rat monoclonal antibody specific for the α 5 chain of collagen IV. It is useful in Western blot and indirect immunofluorescence experiments. Product specifications	Specification	Detail Anti Collagen 4 (Alpha 5) mAb (Clone H52) Cat No \| SGE-C452 Application \| Staining of human cryostat sections by indirect immunofluorescence (acid-urea treatment is required) and Western blotting (dilution x10) Quantity \| 0.5 ml Appearance \| Solution Purification \| Hybridoma cell supernatant was diluted with culture medium containing 10% fetal bovine serum and 0.1% NaN3. Fluorescent labeling \| none Clone name \| H52 (rat IgG2b, kappa) Specificity \| H52 is specific to NC1 domain of α 5(IV) (Reference 1). Reactive with NC1 domain of α 5(IV) of rat, rabbit, guinea pig, dog, and cow. (Reference 1) Antibodies preparation \| Monoclonal antibody was prepared by the rat lymph node method developed by Shigei Medical Research Institute with a synthetic peptide of type IV collagen as immunogen. Storage \| Store below -30° C. Stable for several years under this condition. Source \| Shigei Medical Research Institute, 2117 Yamada, Okayama 701-0202, Japan; TEL: +81-86-282-3113; FAX: +81-86-282-3115; E-mail: inst@shigei.or.jp References Kagawa M et al. Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport syndrome. Nephrol. Dial. Transplant. 12: 1238-1241 (1997) (PMID: 9198058) Borza DB et al. The NCI domain of collagen IV encodes a novel network composed of the α 1, α 2, α 5, and α 6 chains in smooth muscle basement membranes. J. Biol. Chem. 276: 28532-28540 (2001) (PMID: 11375996) Sado et al. Establishment by the rat lymph node method of epitope-defined monoclonal antibodies recognizing the six different α chains of human type IV collagen. Histochem. Cell Biol. 104: 267-275 (1995) (PMID: 8548560) - Yoshioka K et al. Type IV collagen α 5 chain: Normal distribution and abnormalities in X-linked Alport syndrome revealed by monoclonal antibody. Am. J. Pathol. 144: 986-996 (1994) (PMID: 8178947) - Ninomiya Y et al. Differential expression of two basement membrane collagen genes, COL4A6 and COL4A5, demonstrated by immunofluorescence staining using peptide-specific monoclonal antibodies. J. Cell Biol. 130: 1219-1229 (1995) (PMID: 7657706) - Naito I et al. Relationship between COL4A5 gene mutation and distribution of type IV collagen in male X-linked Alport syndrome. Kidney Int. 50: 304-311 (1996) (PMID: 8807602) Citations - Bu L et al. Somatic Mosaicism in a Male Patient With X-linked Alport Syndrome. Kidney Int Rep. 14(4): 1031-1035 (2019) (PMID: 31312776) - Samar M et al. Negative Staining for COL4A5 Correlates With Worse Prognosis and More Severe Ultrastructural Alterations in Males With Alport Syndrome. Kidney Int Rep. 2(1): 44-52 (2017) (PMID: 29142939) - Malone AF et al. Functional assessment of a novel COL4A5 splice region variant and immunostaining of plucked hair follicles as an alternative method of diagnosis in X-linked Alport syndrome. Pediatr Nephrol. 32(6): 997-1003 (2017) (PMID: 28013382) - Nozu K et al. X-linked Alport syndrome caused by splicing mutations in COL4A5. Am Soc Nephrol. 9(11): 1958-64 (2014) (PMID: 25183659) - Matsubara S et al. Pregnancy complicated with Alport syndrome: a good obstetric outcome and failure to diagnose an infant born to a mother with Alport syndrome by umbilical cord immunofluorescence staining. Obstet Gynaecol Res. 35(6): 1109-14 (2009) (PMID: 20144175) - Patey-Mariaud de Serre N et al. Collagen alpha5 and alpha2(IV) chain coexpression: analysis of skin biopsies of Alport patients. Kidney Int. 72(4): 512-6 (2007) (PMID: 17554254) - Kharrat M et al. Autosomal dominant Alport's syndrome: study of a large Tunisian family. Kidney Dis Transpl. 17(3): 320-5 (2006) (PMID: 16970251)
Clonality	Monoclonal
Fraction	Ascites
Preservative	0.1% NaN3 in LIQ

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

SGE-C452.pdf

SGE-C452-datasheet.pdf

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Menge: 500 ul

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