pROX-FL92.1cggg

Protein Engineering/Cell Free Synthesis/Pinpoint Protein Modification/Expression Plasmids; Protein Engineering/Cell Free Synthesis/Reconstituted/Expression Plasmids

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The pROX expression vector series are for expressing proteins in E. coli cell-free coupled transcription/translation systems, including PURESYSTEM® and The RYTS Kit. Each vector in the series contains optimally positioned upstream T7 promoter and RBS and downstream T7 terminator sequences. In addition, they encode N terminal ProX (see below for explanation) and C terminal hexahistidine tags. Expression templates generated using RYTS Linear Template Kits contain convenient restriction enzyme sites to facilitate subcloning into pROX series expression vectors.
Used in conjunction with Protein Express' s Clover Direct ™ line of tRNA reagents for site-directed protein functionalization, pROX series expression vectors can be used to express proteins containing unnatural amino acids. CloverDirect™ UAG or CGGG aminoacyl tRNAs are available charged with different unnatural amino acids that confer a range of functionality, including fluorescent-labeling, biotin-labeling, PEGylation, chemical crosslinking and photo-regulation. Targeting incorporation of specific unnatural amino acids is achieved by designing protein expression templates with UAG and/or CGGG codons at user-defined positions. The ProX tag is a special N terminal 14 residue peptide designed for the incorporation of certain large unnatural amino acids that require precise N terminal positioning (codon 9) and a specific codon context (flanked by AAC and TCT). Codon 9 of the ProX tag can be varied between UAG, CGGG or TTT (as a control).