Ab-Rapid SPiN Ex-5mL

4°C

Alkali-resistant affinity medium for IgG purification
Ab-Capcher ExTra is an alkali-resistant affinity medium for IgG purification, which is coupled with an alkali-resistant Protein A-derivative (Protein A-R28) designed for higher IgG-binding capacity than that of Ab-Capcher. The dynamic binding capacity of Ab-Capcher ExTra is apporoximately 70 mg human IgG/mL of medium at 100 cm/min. Ab-Capcher ExTra shows higher binding to mouse and rat IgGs exceeding Ab-Capcher. Since Pyrogen testing is carried out on an every lot of manufacturing, antibodies purified with Ab-Capcher ExTra are suitable for cell-based assay*.
*Recommended that endotoxin-free buffers are used for purification of IgG with Ab-Capcher ExTra.
Protein A-R28 is an alkali-tolerant IgG-binding protein derived from protein A, which is developed with ProteNova's patent technology. Protein A-R28 strongly binds to various species and subclasses of IgG, compared with Protein A and G. The coupling to resin (Ab-Capcher) provides an alkali-washable unique affinity medium with high binding capacity for immunoglobulin, which is useful for purification of human, rabbit, and mouse IgGs including mouse IgG1.Ab-Aapcher is also useful for immuno-precipitation experiments.
Feature and Advantages: Dynamic binding capacity (100 cm/hr) is 70 mg/mL.
Reusable by washing with alkali.