TArget Clone-Plus

Nucleic Acid Engineering/Cloning/TA Cloning

-20°C

Convenient TA Cloning System for PCR Products
TArget Clone -Plus- [Code No. TYB-TAK-201] can be applied to the TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. TYB-KOD-201] or KOD FX [Code No. TYB-KFX-101]. TArget Clone -Plus- contains the 10 x A-attachment mix. This reagent is a mixture of anti-KOD DNA polymerase antibody1) specific to KOD 3'J5' exonuclease activity (proof-reading activity) and Taq DNA polymerase, which exhibits terminal transferase activity. The 10 x A-attachment mix allows blunt end PCR products to acquire overhanging dA at the 3'-ends.
Features: [TArget Clone & TArget Clone -Plus-]
- Ligation step can be completed in 5 min.
- PCR products can be used without purification.
-The vector generates blue E. coli colonies on X-gal/(IPTG) plates in the case of no inserts.
- Realizes TA cloning of blunt-end products from KOD -Plus- and KOD FX.
- Addition of dA to the 3'-ends of PCR product can be competed in 10 min at 60 °C.