Avermectins ELISA Kit

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Hersteller Creative Diagnostics
Kategorie
Typ Elisa-Kit
Specific against other
Menge 96T
ArtNr DEIA041
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Abbr
Avermectins Kit
Storage
Unopened Kit: Store at2 - 8oC. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A, TMB Solution B, TMB Stop Solution, Wash Buffer, HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 monthat 2 - 8oC. Microplate Wells: Return unused wells to the foil pouch containing the desiccantpack, reseal along entire edge of zip-seal. May be stored for up to 1 monthat 2 - 8oC.
Reagents And Materials Provided
Microplate: 96 well polystyrene microplate(12 strips of 8 wells) coated with Avermectins, Avermectins Standards (6): 0, 0.5, 1.5, 4.5, 13.5, 40.5 ppb, 6 vials, High concentration Avermectins Standards: 100ppb, 2ml, 1 vial, Antibody Solution: 7ml, 1 vial, HRP Conjugate Antibody: 12ml, 1vial, Sample Diluent(10×): 30 ml. Use to dilute samples. Wash Solution (20×) Concentrate: 40 ml, 1 vial, TMB Solution A: 7ml, 1 vial, TMB Solution B: 7ml, 1 vial, TMB Stop Solution: 7ml, 1 vial, Microtiter plate sealers Plastic Sealable Bag
Precautions
1. The kit should be equilibrated to room temperature (20-23C) beforeopening any vials and starting the assay. It is highly recommended that thesolutions be used as soon as possible after rehydration. 2. Do not mix or substitute reagents with those from other lotsor sources. 3. To avoid cross-contamination, change pipette tips betweenadditions of each standard level, between sample additions, and betweenreagent additions. Also, use separate reservoirs for each reagent. 5. Crystals could appear in the 20X wash solution due to highsalt concentration in the stock solutions. Crystals are readily dissolved atroom temperature or at 37C before dilution of the buffer solutions. 6. Keep TMB Substrate protected from light. 7. The Stop Solution provided with this kit is an acid solution.Wear eye, hand, face, and clothing protection when using this material.
Detection Method
Competitive Enzyme Immunoassay
Sample
tissue and vegetable
Evaluation
The evaluation of the ELISA can be performed using commercialELISA evaluation programs (4-Parameter (preferred) or Logit/Log. For manualevaluation, calculate the mean absorbance value for each of the standards.Calculate the %B/B0 foreach standard by dividing the mean absorbance value for each standard by theZero Standard (Standard 0) mean absorbance. Construct a standard curve byplotting the %B/B0 foreach standard on the vertical linear (y) axis versus the corresponding Avermectinsconcentration on the horizontal logarithmic (x) axis on graph paper. %B/B0 for samples will then yieldlevels in ppb of a Avermectins by interpolation using the standard curve.Samples showing lower concentrations of Avermectins compared to Standard 1 (0.5ng/mL) are considered as negative. Samples showing a higher concentrationthan Standard 5 (40.5 ng/mL) must be diluted further to obtain accurateresults.

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Menge: 96T
Lieferbar: In stock
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