Avermectins ELISA Kit

Avermectins Kit

Microplate: 96 well polystyrene microplate(12 strips of 8 wells) coated with Avermectins, Avermectins Standards (6): 0, 0.5, 1.5, 4.5, 13.5, 40.5 ppb, 6 vials, High concentration Avermectins Standards: 100ppb, 2ml, 1 vial, Antibody Solution: 7ml, 1 vial, HRP Conjugate Antibody: 12ml, 1vial, Sample Diluent(10×): 30 ml. Use to dilute samples. Wash Solution (20×) Concentrate: 40 ml, 1 vial, TMB Solution A: 7ml, 1 vial, TMB Solution B: 7ml, 1 vial, TMB Stop Solution: 7ml, 1 vial, Microtiter plate sealers Plastic Sealable Bag

Competitive Enzyme Immunoassay

The evaluation of the ELISA can be performed using commercialELISA evaluation programs (4-Parameter (preferred) or Logit/Log. For manualevaluation, calculate the mean absorbance value for each of the standards.Calculate the %B/B0 foreach standard by dividing the mean absorbance value for each standard by theZero Standard (Standard 0) mean absorbance. Construct a standard curve byplotting the %B/B0 foreach standard on the vertical linear (y) axis versus the corresponding Avermectinsconcentration on the horizontal logarithmic (x) axis on graph paper. %B/B0 for samples will then yieldlevels in ppb of a Avermectins by interpolation using the standard curve.Samples showing lower concentrations of Avermectins compared to Standard 1 (0.5ng/mL) are considered as negative. Samples showing a higher concentrationthan Standard 5 (40.5 ng/mL) must be diluted further to obtain accurateresults.