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Recombinant Human Transcription factor BTF3(BTF3),partial

Recombinant Human Transcription factor BTF3(BTF3),partial

Hersteller	Cusabio
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Specific against	other
Format	Liquid or Lyophilized powder
Menge	1mg
Host	E.coli
ArtNr	CSB-RP017144h-1
Konjugat/Tag	GST
eClass 6.1	34160400
eClass 9.0	42020190
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Research Areas	Transcription
Uniprot ID	P20290
Gene Names	BTF3
Organism	Homo sapiens (Human)
AA Sequence	TIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTAT ADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIH FNNPKVQASLAANTFTITGHAETKQLTEMLPSILN QLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDD DEVPDLVENFDEASKNEAN
Expression Region	48-206aa
Sequence Info	Partial
Source	E.coli
Tag Info	N-terminal GST-tagged
MW	44.3 kDa
Alternative Name(s)	Nascent polypeptide-associated complex subunit beta ; NAC-betaRNA polymerase B transcription factor 3
Relevance	When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they erge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Reference	Sequencing and expression of complementary DNA for the general transcription factor BTF3.Zheng X.M., Black D., Chambon P., Egly J.-M.Nature 344:556-559(1990)
Purity	Greater than 90% as determined by SDS-PAGE.
Form	Liquid or Lyophilized powder
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Subcellular Location	Cytoplasm, Nucleus
Protein Families	NAC-beta family
Tag Information	N-terminal GST-tagged

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