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Recombinant Human Napsin-A(NAPSA)

Hersteller Cusabio
Kategorie
Typ Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Menge 100ug
Host E.coli
ArtNr CSB-EP015452HU-100
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Research Areas
Cell Biology
Uniprot ID
O96009
Gene Names
NAPSA
Organism
Homo sapiens (Human)
AA Sequence
KPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTG SSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQAN GTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFG EALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPM DVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGS DPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTL CAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPL LAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYV IQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFL GTYVAVFDRGDMKSSARVGLARARTRGADLGWGET AQAQFPG
Expression Region
64-420aa
Sequence Info
Full Length of Mature Protein
Source
E.coli
Tag Info
N-terminal 6xHis-tagged
MW
42.5 kDa
Alternative Name(s)
Aspartyl protease 4 ; ASP4 ; Asp 4Napsin-1TA01/TA02
Relevance
May be involved in processing of pneumocyte surfactant precursors.
Reference
Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity.Yan R., Bienkowski M.J., Shuck M.E., Miao H., Tory M.C., Pauley A.M., Brashier J.R., Stratman N.C., Mathews W.R., Buhl A.E., Carter D.B., Tomasselli A.G., Parodi L.A., Heinrikson R.L., Gurney M.E.Nature 402:533-537(1999)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
May be involved in processing of pneumocyte surfactant precursors.
Subcellular Location
Secreted
Protein Families
Peptidase A1 family
Tissue Specificity
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
Tag Information
N-terminal 6xHis-tagged

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ug
Lieferbar: In stock
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