ArtNr |
CSB-EP356214BRJ-1 |
Hersteller |
Cusabio
|
Menge |
1 mg |
Quantity options |
1 mg
10 ug
100 ug
20 ug
200 ug
50 ug
500 ug
|
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid |
Specific against |
other |
Host |
E.coli |
Konjugat/Tag |
SUMO, HIS |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Sequence |
AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGI DSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG TIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYS WIINGIEWAISNNMDVINMSLGGPTGSTALKTVVD KAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTI AVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLP GGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQ VRD |
Protein Familie |
Peptidase S8 family |
Citations |
Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation.Stahl M.L., Ferrari E.J. Bacteriol. 158:411-418(1984) |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Manufacturer - Conjugate / Tag |
N-terminal 6xHis-SUMO-tagged |
Storage Conditions |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Molecular Weight |
43.7 kDa |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Relevance |
Subtilisin is an Extracellular domain alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. |
Expression Region |
107-381aa |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. |
Subcellular Location |
Secreted |
Gene Names |
aprE |
Sequence Info |
Full Length of Mature Protein |
Organism |
Bacillus subtilis (strain 168) |
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