Anti-Acetyl Lysine Mouse Monoclonal Antibody HRP Conjugates (Trial size)

Mouse / IgG1

On Arrival: 4°C

AAC03-HRP, is an HRP labeled pan-acetyl lysine mouse monoclonal antibody that is part of the Signal-Seeker product line. AAC03-HRP anti-acetyl-lysine antibody, clone 19C4B2.1, has been tested in western blot applications and has been shown to be at least 10 times more potent than using unconjugated AAC03 with standard mouse-HRP secondary. The AAC03, anti-acetyl lysine antibody recognizes proteins post-translationally modified by acetylation on the epsilon amine groups of lysine residues that occur on 30-50% of all proteins and in particular histones, p53, tubulin and myosin. A proprietary mixture of acetylated proteins was used to produce the 19C4B2.1, highly robust antibody that has been shown to recognize a wide range of acetylated proteins.

AB_2884961

Fig 1: A: Murine tissue extract, 30 ?g brain extract. B: 30 ?g of Cos-7 cell lysate treated with TSA and nicotinamide (+) or untreated (-). Strongly enhanced bands at 55 and 14-16 kDa in TSA-treated lysate correspond to acetylated tubulin and histone proteins, respectively. C: Titration of acetylated BSA. Lanes 1-5 contain 0.5, 0.1, 0.05, 0.01, and 0.005 ng Ac-BSA, lanes 6-7 contain 500 and 1000 ng non-acetylated BSA, respectively. AAC03-HRP was used at a 1:3000 dilution following the recommended western blot protocol. To see the full Western blot comparison, see the Optimized Protocols or the product datasheet. Acetylation of proteins can occur as a co-translational or post-translational modification (PTM) (1). Co-translational acetylation occurs at the N-terminal of approximately 85% of mammalian proteins, it is irreversible and is thought to be important in protein stability, localization and synthesis (1). Post-translational acetylation occurs on the epsilon amino group of lysine residues as a reversible and highly dynamic PTM that is known to be a key regulator in multiple cellular events, including chromatin structure, transcription, metabolism, signal transduction and cytoskeletal regulation (2-3). To date over 4, 000 proteins have been identified as targets for PTM acetylation which is comparable to phosphorylation in cellular prevelance (3). Antibody AAC01 detects acetyl lysine PTMs.