Rac1 G-LISA Activation Assay Kit (colorimetric)

The proprietary Rac1 G-LISA Activation Assay that is faster, easier and more precise than traditional pull-down methods to measure Rac1 Activation.

• Specific for Rac1
• Colorimetric Based
• Fast Results
• Extremely Sensitive

General Information

This Rac1 G-LISA is a colorimetric based assay and shares the many advantages that all G-LISA activation assay have. This assay is specific for Rac1.

The Rac1 G-LISA kit contains a Rac-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Rac1 is captured by this protein while inactive GDP-bound Rac1 is removed following washes. The active Rac1 bound to the wells is detected with a Rac1 specific antibody. The degree of Rac1 activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.

Figure 1.The simple procedure of the Rac1 G-LISA assay.

Kit Contents - Enough reagents for 96 assays (Stripwell)

• 96 individual Rac1-GTP binding wells
• Anti-Rac1 antibody (Cat. # ARC03)
• Secondary Antibody - HRP
• Rac1 control protein
• Cell Lysis Buffer
• Wash buffer
• Antigen Presenting Buffer
• Antibody Dilution Buffer
• HRP Detection and Stop Reagents
• Precision Red Advanced Protein Assay (Cat. # ADV02)
• Protease Inhibitor Cocktail (Cat. # PIC02)

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Figure 2. Rac1 activation by EGF measured by G-LISA. Swiss 3T3 cells were serum starved (SS) for 48 h and treated with EGF (10 ng/ml for 2 min). 12.5 ?g and 25 ?g of cell lysates were subjected to the G-LISA assay. Absorbance was read at 490 nm.