Question 1: What is the chemical nature of the Rho activator CN01?
Answer 1: CN01 is calpeptin and has the peptide sequence of Z-Leu-Nle-CHO. The molecular weight is 362.5 and the CAS number is 117591-20-5. Calpeptin is supplied as a lyophilized white solid and each vial contains 1 mg (10 units) of CN01. The purity is &ge, 95% as determined by HPLC.
Question 2: Does CN01 selectively activate Rho?
Answer 2: No, CN01 (calpeptin) does not specifically activate Rho. Calpeptin-induced Rho activation is indirect via a mechanism involving inhibition of Shp-2 phosphatase. Inhibition of Shp-2 allows constitutive activation of Rho GEFs. CN01 also inhibits calpain-1 in a mechanism that is unrelated to Rho activation. For a direct and specific Rho activator, please see Cat. # CN03.
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