FtsZ Protein (E.coli recomb. no tag)

Question 1: Are other FtsZ proteins available besides the FtsZ from E. Coli?

Answer 1: Yes, Cytoskeleton offers custom protein purification for FtsZ proteins. Or we can align the gene sequences of the FtsZ proteins we currently have in our regular product line to see which protein is the closest homolog to your FtsZ protein of interest. We recommend using a FtsZ protein that has the closest homology to your target FtsZ protein. While the function of the FtsZ proteins from different bacteria may be the same, there are differences in sequence homology between FtsZ proteins across bacterial lines, strongly suggesting that each FtsZ protein will have a unique pharmacological profile in response to drug treatment. In addition, different FtsZ proteins are likely to have different binding proteins (or at least bind at different affinities) and FtsZ protein dynamics are likely to differ as well. For these reasons, we recommend using FtsZ proteins that match your FtsZ protein of interest in regard to sequence homology.

Question 2: What is the proper way to store the FtsZ protein (Cat. #FTZ01)?

Answer 2: The lyophilized protein should be stored at 4°C with fresh desiccant where it will be stable for 6 months. Storage at -70°C is also an option, though the rubber seal on the container can crack at this temperature and allow in moisture. Therefore we recommend storing at 4°C. If stored at -70°C, it is imperative to include desiccant with the lyophilized protein if this storage condition is utilized. After reconstituting as directed, the concentrated protein should then be aliquoted and snap frozen in liquid nitrogen. Store these aliquots at -70°C for 6 months. For working concentrations, further dilution of the protein should be made with MES Buffer (100 mM MES pH6.5, 1mM EGTA). NOTE: It is very important to snap freeze the FtsZ protein in liquid nitrogen as other methods of freezing will result in significantly reduced activity. Defrost rapidly by placing in a room temperature water bath for 1 min. Avoid repeated freeze/thaw cycles.