Vergleich

RhoC His Protein: wild type

ArtNr RH03-A
Hersteller Cytoskeleton
Menge 1 x 100 ug
Kategorie
Typ Proteins
Specific against other
Purity Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-RhC samples are ca.90% pure.
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ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Small G protein, RhoC, RhC protein, Rho C, HIS-RhoC
Similar products Rho C, Small G protein, RhoC, HIS-RhoC, RhC protein
Versandbedingung Raumtemperatur
Lieferbar
Shipping Temperature
AT
Storage Conditions
On Arrival: 4°C
Delivery Time
1-2 Weeks
Weight (grams)
7
Product Uses
RhC biochemistry
RhC GTPase assays
RhC nucleotide exchange assays
RhC binding studies
Material
The human RhC protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
The recombinant protein is approximately 30 kDa, consisting of the RhC protein plus a histidine tag in the amino-terminus.
For other forms of Rho proteins as well as many other purified small G-proteins, see our main small G-protein product page.
Biological Activity
The biological activity of His-RhC is determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to sequester magensium ions from the His-RhC protein, thereby stimulating nucleotide exchange activity. The RhoGEF exchange assay biochem kit (Cat. # BK100) is used to monitor the exchange ability of His-RhoC.
Figure 1 Legend
Figure 1: His-RhC protein purity determination. A 10 ug sample of RH01 (His-RhC molecular weight approx. 30 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 x 100 ug
Lieferbar: In stock
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