Comparison

RhoC His Protein: wild type

Item no. RH03-A
Manufacturer Cytoskeleton
Amount 1 x 100 ug
Category
Type Proteins
Specific against other
Purity Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-RhC samples are ca.90% pure.
Citations Coming soon!  For the most recent publications citing this product, please contact our Technical Service department at infohoelzel.de
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Small G protein, RhoC, RhC protein, Rho C, HIS-RhoC
Similar products Rho C, Small G protein, RhoC, HIS-RhoC, RhC protein
Shipping Condition Room temperature
Available
Shipping Temperature
AT
Storage Conditions
On Arrival: 4°C
Delivery Time
1-2 Weeks
Weight (grams)
7
Product Uses
RhC biochemistry
RhC GTPase assays
RhC nucleotide exchange assays
RhC binding studies
Material
The human RhC protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
The recombinant protein is approximately 30 kDa, consisting of the RhC protein plus a histidine tag in the amino-terminus.
For other forms of Rho proteins as well as many other purified small G-proteins, see our main small G-protein product page.
Biological Activity
The biological activity of His-RhC is determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to sequester magensium ions from the His-RhC protein, thereby stimulating nucleotide exchange activity. The RhoGEF exchange assay biochem kit (Cat. # BK100) is used to monitor the exchange ability of His-RhoC.
Figure 1 Legend
Figure 1: His-RhC protein purity determination. A 10 ug sample of RH01 (His-RhC molecular weight approx. 30 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 x 100 ug
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?