Arp2/3 Protein Complex (Porcine brain)

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The Arp2/3 protein complex consists of 7 protein subunits, all present in approximately equal stoichiometry (see Figure 1). The Arp2/3 complex is a key regulator of actin filament nucleation and the protein subunits are highly evolutionarily conserved. The protein complex has been purified from porcine brain and is supplied as a lyophilized powder.When reconstituted with distilled water, the complex is in the following buffer: 20 mM Tris pH 7.5, 25 mM KCl, 1 mM MgCl₂ 0.5 mM EDTA, 0.1 mM ATP, 1% dextran and 5% sucrose. The molecular weight of the Arp2/3 complex is 224 kDa.
Note: RP01P from procine brain replaces RP01 from bovine brain. We have compared the activity of Arp2/3 complex from both sources and have found them to behave identically in actin polymerization assays (see Fig. 2)

Figure 1. Arp2/3 Protein Purity Determiniation: A 10 ?g sample of Arp2/3 complex was separated by electro-phoresis in a 4-20% SDS-PAGE system and stained with Coomassie Blue. Protein quantita-tion was determined with the Precision RedTM Protein Assay Reagent (Cat. # ADV02). Mark12 molecular weight markers are from Life Technologies.