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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

ArtNr	ELS-E-BC-K020-M
Hersteller	Elabscience
Menge	96 T
Quantity options	500 Assays 96 T 96 T 48 T
Kategorie	Cell Biology Cell Metabolism Other Assays & Kits Other Kits
Typ	Kit
Specific against	other
Sensitivity	0.2 U/mL
Citations	https://www.sciencedirect.com/science/article/pii/S2452199X23000154 https://pubs.acs.org/doi/abs/10.1021/acsnano.9b09974 https://www.sciencedirect.com/science/article/pii/S0304389421020707 https://www.sciencedirect.com/science/article/pii/S2090123222001229 https://pubs.acs.org/doi/abs/10.1021/acsami.1c14201 https://cmbl.biomedcentral.com/articles/10.1186/s11658-023-00416-1 https://www.sciencedirect.com/science/article/pii/S004896972105405X https://www.mdpi.com/1983336 https://www.mdpi.com/1905716 https://www.sciencedirect.com/science/article/pii/S0925400520305839 https://www.mdpi.com/article/10.3390/ijms232315400 https://www.nature.com/articles/s41419-020-02777-3
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	T-SOD
Versandbedingung	Gekühlt
Gefahren-Info	Comply with cargo safety certificate
Lieferbar
Manufacturer - Category	Cell Metabolism Assays
Shipping Temperature	Ice bag
Storage Conditions	This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Research Areas	Oxidative stress, Plant Physiology Series, Ferroptosis
Description	The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
Assay time	30 min
Valid time	196
Old Cat No.	E-BC-K020
Detection Type	Colorimetric method
Tested Sample Types	serum; plasma; hydrothorax; ascites; urine; cells; tissue
Detection Instrument	Microplate reader
Test Principle	The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
Result Type	Enzyme Activity
Inter cv	3.7%
Intra cv	2.9%
Category ID III	Oxidative Stress; Plant Physiology Series; Ferroptosis

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