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Recombinant Human NEDD8 Protein (His Tag, SUMO Tag)

Recombinant Human NEDD8 Protein (His Tag, SUMO Tag)

ArtNr	ELS-PKSH032791-50ug
Hersteller	Elabscience
Menge	50 ug
Quantity options	10 ug 50 ug
Kategorie	Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Autophagy
Typ	Proteins
Format	Lyophilized Powder
Specific against	Human (Homo sapiens)
Host	E.coli
Konjugat/Tag	HIS, SUMO
Purity	> 95 % as determined by reducing SDS-PAGE.
Sequence	Met 1-Gly76
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein Nedd8
Similar products	NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein Nedd8
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	Recombinant Proteins / Others
Manufacturer - Conjugate / Tag	N-His-SUMO
Shipping Temperature	This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight	20.9 kDa
Observed Molecular Weight	24 kDa
Manufacturer - Research Areas	Cell biology
Background	Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Abbreviation	NEDD8
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Reconstitution	Please refer to the printed manual for detailed information.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Activity	Not validated for activity

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

ELS-PKSH032791-50ug-datasheet.pdf

ELS-PKSH032791-50ug-safety-datasheet.pdf