Bull 4MID kit (ELISA Kit)

Bull 4MID kit (ELISA Kit)

+4°C

The Bull 4MID® KIT is the first ELISA assay that objectively validates semen quality by quantifying a protein biomarker called proAKAP4 in sperm samples (in ejaculate, chilled or frozen semen in extender or in isolated spermatozoa).

Quantitative

Ejaculates, semen in extenders, Isolated spermatozoa)

From one to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per 96 well microplate / per kit)

4 ng / mL

Bastan I and Akcay E (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker. Andrologia e14164. https:// doi.org/10.1111/and.14164
Ruelle I, Sergeant N, Bencharif D, Charreaux F, Thorin C, Michaud S, Dordas-Perpinyà M, Jouy N, Audry S, Maurage CA, Delehedde M and Briand-Amirat L (2020) ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):145.
Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448
Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8:44-45.
Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
Fu Q, Pan L, Huang D, Wang Z, Hou Z and Zhang M (2019) Proteomic profiles of buffalo spermatozoa and seminal plasma. Theriogenology. Vol. 134:74-82.
Ruelle I, Charreaux F, Bencharif D, Thorin C, Michaud S, Schmitt E, Sergeant N, Briand-Amirad L and Delehedde M (2019) Assessment of the sperm specific protein proAKAP4 as a marker to evaluate sperm quality and fertility in Holstein bulls. Rev. Bras. Reprod. Anim. Vol.43(2):472.
Peddinti D, Nanduri B, Kaya A, Feugang JM, Burgess SC and Memili E (2008) Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility. BMC Systems Biology. Vol. (2)19:1-13.

- 96-well microplate reader measuring absorbance at 450 nm
- Horizontal orbital microplate shaker (300 rpm)
- Vortex
- Multichannel pipette of 300 μL
- Pipettes of 20 μL, 200 μL and 1000 μL
- Pipette tips
- Polypropylene microtubes of 1.5 mL (for sample preparation)
- One polypropylene tube of 15 mL (for R6 dilution)
- Plastic reagent reservoirs
- One glass bottle (for R2 dilution)
- Scissors
- Pen
- Aluminum foil
- Ultrapure or double deionized water