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Human LILRB2 ELISA Kit PicoKine®

Human LILRB2 ELISA Kit PicoKine®

ArtNr	BOS-EK2124
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Quantity options	96 wells/kit, with removable strips.
Kategorie	Neuroscience Aging & Neurological Disorders ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<50 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Leukocyte immunoglobulin-like receptor subfamily B member 2,LIR-2,Leukocyte immunoglobulin-like receptor 2,CD85 antigen-like family member D,Immunoglobulin-like transcript 4,ILT-4,Monocyte/macrophage immunoglobulin-like receptor 10,MIR-10,CD85d,LILRB2,ILT4,LIR2,MIR10
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	LILRB2
Gene Full Name	leukocyte immunoglobulin like receptor B2
Manufacturer - Specificity	Natural and recombinant Human LILRB2
Background	Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Immunogen	Expression system for standard: NS0; Immunogen sequence: Q22-V461
Protein Name	Leukocyte immunoglobulin-like receptor subfamily B member 2
Protein Function	Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Subcellular Localization	Cell membrane. Single-pass type I membrane protein.
Description	Human LILRB2 ELISA Kit PicoKine® (96 Tests). Quantitate Human LILRB2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed in monocytes and at lower levels in myeloid and plasmacytoid dendritic cells. Expressed in tolerogenic IL10-producing dendritic cells. Expressed in myeloid-derived suppressor cells during pregnancy. Detected at low levels in natural killer (NK) cells. Expressed in B cells.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 \| 20000 O.D. \| 0.055 \| 0.125 \| 0.169 \| 0.299 \| 0.545 \| 0.956 \| 1.437 \| 2.064
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 384 \| 2313 \| 8445 \| 492 \| 2436 \| 9458 Standard deviation \| 17.28 \| 131.84 \| 388.47 \| 25.09 \| 143.72 \| 699.89 CV (%) \| 4.5% \| 5.7% \| 4.6% \| 5.1% \| 5.9% \| 7.4%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 384 \| 401 \| 375 \| 392 \| 388 \| 9.61 \| 2.4% Sample 2 \| 2313 \| 1997 \| 2156 \| 1982 \| 2112 \| 134.59 \| 6.3% Sample 3 \| 8445 \| 7435 \| 7826 \| 7234 \| 7735 \| 461.89 \| 5.9% *number of samples for each test n=16.
Capture antibody and Detection antibody	a monoclonal antibody from mouse\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Alias	Leukocyte immunoglobulin-like receptor subfamily B member 2, LIR-2, Leukocyte immunoglobulin-like receptor 2, CD85 antigen-like family member D, Immunoglobulin-like transcript 4, ILT-4, Monocyte/macrophage immunoglobulin-like receptor 10, MIR-10, CD85d, LILRB2, ILT4, LIR2, MIR10
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK2124.pdf

BOS-EK2124-datasheet.pdf

BOS-EK2124-safety-datasheet.pdf