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Mouse sE-Selectin ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Mouse sE-Selectin ELISA Kit EZ-Set™ (DIY Antibody Pairs)

ArtNr	BOS-EZ0502
Hersteller	Boster
Menge	5 plates/kit
Quantity options	5 plates/kit
Kategorie	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience By Cell Type Hematology Leukemia Cell Biology Cell Movement Cell Adhesion ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<10 pg/ml
Sequence	Belongs to the selectin/LECAM family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	E-selectin,CD62 antigen-like family member E,Endothelial leukocyte adhesion molecule 1,ELAM-1,Leukocyte-endothelial cell adhesion molecule 2,LECAM2,CD62E,Sele,Elam-1
Lieferbar
Manufacturer - Category	EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	66750 MW
Gene Name	SELE
Gene Full Name	E-selectin
Manufacturer - Specificity	Natural and recombinant mouse sE-Selectin
Background	E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.
Immunogen	Expression system for standard: NS0; Immunogen sequence: W22-P557
Protein Name	E-selectin
Protein Function	Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
Subcellular Localization	Cell membrane; Single-pass type I membrane protein.
Description	Mouse sE-Selectin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse SELE in cell culture supernatants, serum and plasma (heparin, citrate).
Sample Type	cell culture supernatants, serum and plasma (heparin, citrate).
Sequence Similarities	Belongs to the selectin/LECAM family.
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 O.D. \| 0.013 \| 0.041 \| 0.080 \| 0.201 \| 0.368 \| 0.751 \| 1.474 \| 2.156
Kit Components	Catalog number \| Description \| Quantity \| Storage of opened/reconstituted material EZ0502-CA \| Rat anti- mouse Sele monoclonal antibody (Capture Antibody) \| 500 µ l, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0502-DA \| Biotinylated goat anti- mouse Sele polyclonal antibody (Detection Antibody) \| 500 µ l, 9.5 µ g/mL (recommended dilution 1:100) AR1103 \| Avidin-Biotin-Peroxidase Complex (ABC) \| 500 µ l (recommended dilution 1:100) EK0502-ST \| Lyophilized recombinant mouse Sele standard \| 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
Anticoagulant	heparin or citrate
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-EZ0502-datasheet.pdf

BOS-EZ0502-safety-datasheet.pdf