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Human VEGF PicoKine® Quick ELISA Kit Europäischer Partner

ArtNr BOS-FEK0539
Hersteller Boster
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity <1 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias VEGFA|MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
Lieferbar
Specificity Natural and recombinant Human VEGFA
Manufacturer - Category
Fast ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Manufacturer - Gene Name
CST3
Gene Full Name
vascular endothelial growth factor A
Sample Type
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Sample Data
Concentration (pg/ml)
Manufacturer - Specificity
Natural and recombinant Human VEGFA
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay PrecisionInter-Assay Precision
Sample
Reproducibility
LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1
Capture antibody and Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
Kit Components
Description|Quantity|Volume
Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
Standard||2
Antibody A|1|3ml
Antibody B|1|3ml
Sample Diluent|1|15ml
TBS-T Wash Buffer (25x)|1|12ml
Color Developing Reagent (TMB)|1|10ml
Stop Solution|1|10ml
Adhesive Plate Sealers|2|Piece
Standard
10 ng/tube
Anticoagulant
heparin, EDTA or citrate
Background
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.
Immunogen
Expression system for standard: NS0; Immunogen sequence: A27-R191
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional)
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Research Category
Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
Protein Name
Vascular endothelial growth factor A
Description
Human VEGF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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