« Back
Home /
Human VEGF PicoKineÂ® Quick ELISA Kit

Human VEGF PicoKineÂ® Quick ELISA Kit

Item no.

BOS-FEK0539

Manufacturer

Boster

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<1 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

VEGFA|MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Available

Specificity

Natural and recombinant Human VEGFA

Manufacturer - Category

Fast ELISA Kits

Storage Conditions

Store at 4Â°C for 6 months, at -20Â°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Manufacturer - Gene Name

CST3

Gene Full Name

vascular endothelial growth factor A

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant Human VEGFA

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity\|Volume Anti-tag Pre-coated 96-well Strip Microplate\|1\|12 strips of 8 wells Standard\|\|2 Antibody A\|1\|3ml Antibody B\|1\|3ml Sample Diluent\|1\|15ml TBS-T Wash Buffer (25x)\|1\|12ml Color Developing Reagent (TMB)\|1\|10ml Stop Solution\|1\|10ml Adhesive Plate Sealers\|2\|Piece
Standard	10 ng/tube
Anticoagulant	heparin, EDTA or citrate
Background	Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.
Immunogen	Expression system for standard: NS0; Immunogen sequence: A27-R191
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 Âµl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Research Category	Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
Protein Name	Vascular endothelial growth factor A
Description	Human VEGF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-FEK0539-datasheet.pdf

BOS-FEK0539-safety-datasheet.pdf