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Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit Europäischer Partner

ArtNr BOS-FEK0977
Hersteller Boster
Menge 96 wells/kit, with removable strips.
Quantity options 96 wells/kit, with removable strips.
Kategorie
Typ Elisa
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Sensitivity <10 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias C-Reactive Protein/CRP,C-Reactive Protein,C-reactive protein,pentraxin-related,CRP,MGC88244,pentraxin 1,PTX1MGC149895
Lieferbar
Manufacturer - Category
Quick ELISA Kits
Storage Conditions
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Gene Name
CST3
Gene Full Name
C-reactive protein, pentraxin-related
Manufacturer - Specificity
Natural and recombinant Mouse Crp
Background
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21, 500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
Immunogen
Expression system for standard: NS0; Immunogen sequence: H20-S225
Manufacturer - Research Category
Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
Protein Name
C-reactive protein
Description
Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Sample Data
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000
O.D. | 0.035 | 0.071 | 0.139 | 0.260 | 0.384 | 0.666 | 1.120 | 1.877
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 254 | 1752 | 6770 | 265 | 1851 | 6930
Standard deviation | 11.93 | 94.6 | 453.59 | 12.98 | 105.5 | 512.82
CV (%) | 4.7% | 5.4% | 6.7% | 4.9% | 5.7% | 7.4%
Reproducibility
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 254 | 243 | 280 | 249 | 256 | 13.82 | 5.4%
Sample 2 | 1752 | 1563 | 1416 | 1662 | 1598 | 97.47 | 6.1%
Sample 3 | 6770 | 5545 | 5924 | 6118 | 6089 | 395.78 | 6.5%
*number of samples for each test n=16.
Capture antibody and Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
Kit Components
Description|Quantity|Volume
Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
Standard||2
Antibody A|1|3ml
Antibody B|1|3ml
Sample Diluent|1|15ml
TBS-T Wash Buffer (25x)|1|12ml
Color Developing Reagent (TMB)|1|10ml
Stop Solution|1|10ml
Adhesive Plate Sealers|2|Piece
Standard
10 ng/tube
Anticoagulant
heparin or EDTA
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional)
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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